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SpK

SpK is a bio-insecticide based on the bacterium Yersinia entomophaga.  The whole organism, toxic metabolites and identified genes all display insecticidal properties when injested by susceptible insect pests. The level of this insecticidal activity compares favourably with commercial bio-rational products.

SpK:

  • Is at least as efficacious as Bt, but against a broader spectrum of insect pests,
  • Will be commercially competitive with Bt
  • May be more persistent on insect food surfaces after application
  • Being yet undeveloped, has substantial potential for future optimisation in a development process
  • At least as the whole organism, qualifies as a suitable component of ‘organic’ insect control regimes.
  • Has no detected mammalian or environmental toxicity concerns that might prejudice product registrations
  • Is not difficult, complicated or expensive to produce by fermentation and may be formulated into dry preparations for packaging, storage and distribution under reasonable ambient conditions. These dry formulations are conveniently dispersed in water for spray or similar application onto surfaces of pest insect food.
     

In addition:

SpK is currently being tested for insecticidal activity on diamond back moth larvae that are resistant to Bt, spinosad or a pyrethroid insecticide. It is expected that SpK will display no cross-resistance to any of these insecticide groups. Therefore SpK can be expected to a valuable tool in resistance prevention or management programmes that involve these insecticides. SPK may complement, replace or compete with other insecticides depending on biological and commercial considerations.

 

Effect of live cells and broth components against grass grub larvae.

 

Y. entomophaga secretes high amounts of a Toxin complex into the culture milieu at temperatures at or below 25oC. The filtrates have shown a high degree of efficacy towards grass grub larvae and diamond back moth
 

Figure: Toxin filtrates against grass grub larvae showing change in colour and exudation of fluids (B and C; healthy is A)
 
 
Toxin filtrates against diamond back moth (DBM)
 
/6mL
Total µg protein
Total µg Toxin
Timea
Culture supernatent
Pure toxin
F6
861
2.7
F7
1323
19.22
F8
966
13.25
F24
3171
240
 
a refer graph
 
The DNA sequence for the toxin has been identified and patented. This means this technology could be used in genetically modified systems (similar to Bt)
 
 
 
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